The Microscopy business group at Carl Zeiss is presenting a new microscopy technology at the Society for Neuroscience Annual Meeting in New Orleans, Louisiana. Lightsheet Z.1 provides biologists with a new method of imaging dynamic processes in living organisms.

Observing life

Biologists can use the new microscopy system to observe the development of entire organisms over several days or more. The extremely low phototoxicity and the integrated incubation enable insights into the differentiation of cell groups without harming the specimen. On large objects, in particular, such as fruit fly or zebrafish embryos, the light sheet microscope delivers more information than established methods of fluorescence microscopy. “The bigger the sample, the more you can get out of it with light sheet microscopy,” says Dr. Pavel Toman?ák from the Max Planck Institute for Molecular Cell Biology and Genetics in Dresden, Germany, describing the benefits of the new method. Lightsheet Z.1 can also be used in marine and cell biology, as well as plant physiology.

New perspectives with Multiview

Lightsheet Z.1 works with an expanded light beam, the light sheet, that illuminates only a thin section of the sample, thus protecting the rest of the specimen. Images are captured at a 90 degree angle to the light sheet. Therefore, Lightsheet Z.1 achieves maximum image quality at minimal illumination intensity and is particularly well-suited for long-term examinations of living specimens. Multiview imaging allows data acquisition from different viewing angles. These can be combined through mathematical algorithms into 3D reconstructions and time-lapse videos.

The light sheet system of Lightsheet Z.1 uses a new type of optical concept that combines cylindrical lens optics with laser scanning. Users receive homogeneously illuminated optical sections of complete examination objects.

Dr. Olaf Selchow, Product Manager for Light Sheet Microscopy at Carl Zeiss, states: “I’m confident that this illumination principle is going to revolutionize 3D fluorescence imaging.”

For more information, please visit: www.zeiss.com/lightsheet

Carl Zeiss

The Carl Zeiss Group is an international leader in the fields of optics and optoelectronics. In fiscal year 2010/11 the company’s approx. 24,000 employees generated revenue of about 4.237 billion euros. In the markets for Industrial Solutions, Research Solutions, Medical Technology and Consumer Optics, Carl Zeiss has contributed to technological progress all over the world for more than 160 years. With its innovative technologies and leading-edge solutions, Carl Zeiss is successful in the fields of Semiconductor Manufacturing Technology, Industrial Metrology, Microscopy, Medical Technology, Vision Care and Consumer Optics/Optronics. Carl Zeiss AG is fully owned by the Carl Zeiss Stiftung (Carl Zeiss Foundation).