A new instrument is now available that offers fast transfer of proteins from polyacrylamide gels to typical Western blot membranes, for subsequent antibody detection, speeding up Western blot methods. Thermo Fisher Scientific will showcase these solutions during Analytica 2014 (Hall B1, Booth #101) being held at Messe München in Munich from April 1-4.

The Thermo Scientific Pierce G2 Fast Blotter provides researchers a tool to perform rapid semi-dry transfer of 10-350kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes, for subsequent Western blot detection. When used with the Thermo Scientific Pierce1-Step Transfer Buffer, the Pierce G2 Fast Blotter transfers proteins in 5-10 minutes without the need for gel pre-equilibration. This significant reduction in protein transfer time is accomplished by optimizing the ionic strength of the transfer buffer and increasing the current [amps (A)/cm2] flowing through the transfer stack.

The Pierce G2 Blotter has an easy-to-use color LCD touchscreen with pre-programmed transfer methods that allow users to quickly create, run and save custom transfer methods. The system has been verified to work with commonly used pre-cast and homemade SDS-PAGE gels and can be used to simultaneously transfer up to four mini-sized gels or two midi-sized gels.

The Pierce G2 Blotter is the most recent addition to the Thermo Scientific line of Western blot instruments and reagents, which includes the Thermo Scientific MYECL Imager for Western blot capture and analysis and the popular Thermo Scientific SuperSignal Substrates.

For more information on the new Pierce G2 Fast Blotter and related consumables, please visit www.thermoscientific.com/g2blotter.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science, with revenues of $17 billion and 50,000 employees in 50 countries. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity. Our four premier brands – Thermo Scientific, Life Technologies, Fisher Scientific and Unity Lab Services – offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive support. For more information, please visit www.thermofisher.com.

Contact:

Suzanne Clancy

Thermo Fisher Scientific

Carlsbad, Calif., U.S.

+1 760 602 4545

[email protected]