Optimized and Functionally Tested Enzyme System For Hepatocyte Isolation

The NEW Worthington Hepatocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system.

By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes.

In addition, Worthington use-tests each lot by isolating hepatocytes from adult rats to assure performance, reliability, and consistent yield of viable cells.

Each kit includes single-use enzyme vials, balanced salt solution, buffer, culture media and an optimized protocol for five separate adult rat liver perfusions. A modified protocol for mouse applications is also available.

The method is based on that described by Berry, M.N., modified by Seglen, P.O., and further optimized in conjunction with several collaborating researchers.