Spheroid Imaging Mountant

Shepshed, LEICS., UK – The thermo-reversible mountant CyGEL™ (Biostatus Ltd., UK) enables high resolution imaging of live 3D tumor spheroids cultured in low-adherence conditions and without fixation or cytospin centrifugation. A recent paper by Fredika M. Robertson, et al., Imaging and Analysis of 3D Tumor Spheroids Enriched for a Cancer Cell Phenotype. J Biomol Screen. 2010 15:820-829, confirms that CyGEL™ stabilizes multicellular structures for imaging and characterization, whilst retaining cellular viability, morphology and protein expression. CyGEL™ is optically inert and clear, compatible with fluorescence and permits delivery of organelle-specific and viability probes. Objects can be isolated, imaged and then easily recovered for orthogonal analysis. CyGEL™ allows a breadth of advanced microscopic techniques for live-cell imaging of both mammalian and non-mammalian structures.

Hyperlink: https://www.biostatus.com/news/high_quality_tumor-spheroid_imaging/

Spheroid Imaging Mountant

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