Today atthe 48th annual meeting of The American Society for Cell Biology (ASCB), PerkinElmer, Inc., a global leader focused on improving the health and safety of people and the environment, today announced that it has expanded its leading AlphaLISA® “No Wash” assay line.

The rapidly growing portfolio – now comprising 27 kits – includes kits for detecting key biomarkers associated with inflammation, cancer, neurodegeneration, metabolic disorders and angiogenesis. With AlphaLISA assays, it is now possible to culture cells and detect key analytes using an “All-in-One-Well” format. The kits can be run in a range of complex biological fluids without the need for arduous separation and wash steps. The AlphaLISA technology saves time and eliminates labor intensive processes that are difficult to automate and which reduce precision and can disrupt weak biomolecular interactions.

“The new ‘All-in-One-Well’ capability greatly enhances the AlphaLISA product line and is a dramatic step forward in simplifying cell-based assays,” said Richard Eglen, Ph.D., president, Bio-discovery, PerkinElmer. “AlphaLISA is a robust technology for improving laboratory workflows, eliminating the need for costly washing apparatus and ELISA techniques, as well as facilitating high throughput automation systems.”

In addition to the inflammation, cancer and metabolic disease assays, the “All-in-One-Well” kits now comprise a panel of assays that enables drug development in several critical disease areas. Furthermore, numerous kits allow researchers to detect host cell contamination in biological preparations for therapeutic applications.

The growing panel of AlphaLISA immunoassay kits* includes:

Amyloid beta 40 Alzheimer’s

Amyloid beta 42 Alzheimer’s

CHO Host Cell Proteins Biological

Human IgG Biological

NS0 Host Cell Proteins Biological

Epidermal Growth Factor receptor Cancer

EPO Cancer

Prostatic Specific Antigen Cancer

VEGF Cancer

Cartilage Oligomeric Matrix Protein Inflammation

Granulocyte-Colony Stimulating Factor Inflammation

Granulocyte-Monocyte Colony Stimulating Factor Inflammation

Interferon gamma Inflammation

Interleukin 10 Inflammation

Interleukin 17 Inflammation

Interleukin 1b Inflammation

Interleukin 2 Inflammation

Interleukin 3 Inflammation

Interleukin 6 Inflammation

Interleukin 8 Inflammation

Adiponectin Metabolism

Growth Hormone Metabolism

Glucagon-Like Peptide 1 Metabolism

Insulin Metabolism

Leptin Metabolism

Prolactin Metabolism

P24 Virology

AlphaLISA is a proprietary homogenous bead-based assay that is validated with PerkinElmer’s EnVision® Multiplate Label Readers, a highly flexible modular system that detects several assay formats, including TR-FRET, luminescence and fluorescence.