New AOAC-RI PTM Certification for STEC Detection Workflow Provides Beef Producers with a Rapid but Simple Solution to Screen for STEC
Real-Time PCR workflow gains AOAC-RI approval for rapid and simultaneous detection of E.coli O157:H7 and the Big Six Shiga toxigenic E. coli in raw beef samples
BASINGSTOKE, United Kingdom–Beef producers can simultaneously and accurately screen for Escherichia coli O157:H7 and the Big Six Shiga toxigenic E. coli (STEC) in 375g raw beef samples, enabling rapid product release.
Thermo Fisher Scientific, a market leader in culture media, microbiology, molecular and mass spectrometry systems, has been awarded AOAC-RI Performance Tested Methods SM certification for its Applied Biosystems RapidFinder STEC Detection Workflow. Validated for use on the Applied Biosystems 7500 Fast System, the workflow is a two-stage real-time PCR method, designed to screen for and confirm E. coliO157:H7 and the Big 6 serogroups of non-O157 Shiga toxin-producing E. coli, defined by the USDA Food Safety and Inspection Service (FSIS) as adulterants in the American beef industry (serogroups O26, O45, O103, O111, O121 & O1

