SEATTLE, May 22 — Amnis Corporation announced today the
commercial release of its Extended Depth of Field (EDF(TM)) option for the
ImageStream system. This technology breaks through classical depth of field
limitations of high resolution optical systems, thereby improving the
performance of the ImageStream system and enabling new applications
including high speed analysis of fluorescence in-situ hybridization (FISH).
The EDF option can be installed at time of manufacture or in the field to
allow the existing customer base to take advantage of this new capability.
“The EDF option uses a combination of specialized optics and unique
image processing algorithms to project all structures within the cell onto
one crisp plane of focus,” said William Ortyn, Amnis’ Chief Operating
Officer. “Until now this could only be accomplished via conventional
confocal image stacking techniques which are very slow and tend to suffer
from photo bleaching. The ImageStream with EDF is unhindered by photo
bleaching and operates at rates close to 1000 times faster than confocal
microscopy. The resulting imagery and analysis capabilities are
unparalleled in the cell analysis space setting the stage for the
development of fundamentally new research and clinical applications based
on the ImageStream platform.”
“To create the EDF upgrade we combined WaveFront Coded(TM) technology
from [OmniVision-CDM Optics] with our own optimized image deconvolution
algorithms, with striking results,” said David Basiji, Ph.D., Amnis’
President and CEO. “In addition to the optical benefits, the EDF option
triples the data collection rate by allowing the instrument to be operated
with a larger core stream diameter. One of the most important applications
the ImageStream with EDF can now address is FISH in suspension (FISHIS(R)).
Counting FISH spots accurately requires that all spots be in focus, no
matter where they are in the nucleus … EDF technology, combined with the
tremendous speed and sensitivity of the ImageStream system, will make high
throughput FISHIS a reality. With the recent release of IDEAS 3.0 and the
current release of the EDF option, the Company continues to make excellent
progress in advancing the technological capabilities of our product line.
Amnis Corporation, headquartered in Seattle, WA, develops, manufactures
and markets the ImageStream system for high speed imaging of cells in flow.
The system generates over 100,000 high resolution cell images per minute
with fluorescence sensitivity better than standard flow cytometry. The
ImageStream is supported by the IDEAS(R) image analysis package, a highly
sophisticated yet easy to use tool for processing hundreds of thousands of
images at a time. The software package enables advanced research
applications in hematology, immunology and oncology, and provides a
critical foundation for the company’s clinical applications development.