High Yield Transfection and Baculovirus Production in Insect Cells

[Madison, WI] If low protein expression in insect cells is bugging you, consider the NEW TransIT®-Insect Transfection Reagent. Insect cells are a powerful platform for generating recombinant baculovirus and for producing high-levels of soluble, functional protein. TransIT-Insect is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types with distinct advantages over other reagents such as:

• Exceptional DNA Delivery – In insect cell types including Sf9, High Five™ and S2

• High Baculovirus Production – Ideal for baculovirus expression in insect cells

• Serum Compatibility – Non-liposomal animal-origin free formulation that eliminates media change

• Better Value – Low reagent amounts required per transfection

“Many of the reagents addressing insect cell transfection are cationic-lipid formulations and yield low efficiency in serum containing media,” said Scott Hayes, Vice President, Mirus Bio LLC. “As our customers depend on the non-liposomal nature of TransIT® transfection technologies, they can be assured that TransIT-Insect will meet their expectations for high efficiency and robust protein production.”

In head-to-head testing, transfections with TransIT-Insect show at least 2-fold better protein expression in insect cells.