Tools to Study Genomic Instability and Genotoxic Stress

Trevigen, Inc. is pleased to announce the availability of nineteen DNA repair gene knockdown cell lines,

encompassing the Base Excision Repair pathway. These are target specific LN428 glioblastoma shRNA lentivirus transduced cells that are rigorously qualified and mycoplasma free. The percent knockdown levels range from 75% to 98% as determined by RT-PCR. Lentiviruses are maintained by puromycin selection.

DNA repair proteins maintain the stability of the genome. When repair protein function is impaired

through mutations, the genome may become unstable which is a hallmark of solid tumors. The availability of this panel of knockdown cell lines will permit scientists to study the molecular etiology of tumor genomic instability and to exploit it in oncology research. The first panel covers the Base Excision Repair pathway and is now available from Trevigen, Inc.

APE1 MPG NTHL1 PARG

APE2 MutYH OGG1 SMUG1

BRAC1 NEIL1 PARP1 TDG

ERCC1 NEIL2 PARP2 UNG

MBD4 NEIL3 PARP3 Control Cell

Validation of BER Knockdown Cell Lines: All Trevigen Knockdown Cell Lines are evaluated by RTPCR

and Western Blots when reliable immunoreagents are available.

For more information, visit our web page (link) or contact Trevigen at info@trevigen.com.