Dolomite Bio, a leading provider of single cell technology, today launched a high throughput single nuclei RNA sequencing (sNuc-Seq) protocol for its Nadia Instrument. This latest addition to the Nadia application portfolio enables researchers to cost-effectively profile both complex and archived tissues with the goal of accelerating disease and drug discovery research in areas such as oncology and neurology. The new application is being introduced at the Single Cell Analysis Congress in London on the 8th and 9th of November 2018

Featuring a tailored protocol for nuclei preparation based on the work of Habib et al,¹ the new application enables researchers to consistently and reproducibly capture high numbers of genes at low doublet rates and minimal cost. Detailed information and example data can be downloaded at www.dolomite-bio.com/single-nuclei-rna-seq-nadia

Heike Fiegler, VP of Biology Products at Blacktrace Holdings Ltd, said: “We are delighted to launch sNuc-Seq for Nadia users, which is an important step forward for the single cell community. The new application has significant potential to accelerate disease research into complex tissues, overcoming the problems previously encountered in gene expression profiling of single cells in archived tissues or cells that are generally difficult to isolate.”

To learn more about this new application, join Dolomite Bio’s webinar entitled sNuc-Seq – high throughput RNA profiling of single nuclei with Nadia on the 13th of November 2018, or visit www.dolomite-bio.com/single-nuclei-rna-seq

[1] Habib et al. Massively parallel single-nucleus RNA-seq with DroNc-seq. Nature methods 2017, 14(10) (2017): 955.

About Dolomite Bio

 Dolomite Bio creates innovative products for high throughput single cell research. By encapsulating single cells in microfluidic droplets, our products enable rapid analysis of thousands or millions of individual cells and their biological products.

Dolomite Bio is a brand of Blacktrace Holdings Ltd (www.blacktrace.com), a world leader in Productizing Science®. Our Head Office is based in Royston (near Cambridge), UK and we have regional offices in the USA, Japan, India and Brazil.

Dolomite Bio’s products include the Nadia Product Family, the μEncapsulator System and the RNA Seq system, suitable for use in many applications, such as single cell RNA-Seq, isolating antibody coding sequencing, profiling T cell receptors, droplets for FACS-sorting libraries and encapsulating cells in gel microbeads. To find out more about Dolomite Bio, please visit www.dolomite-bio.com