Hemoglobin enrichment from dried blood spot (DBS) for hemoglobin variant research

MONMOUTH JUNCTION, NJ.– HemoVoid™ Blood Card is developed specifically for the purification & enrichment of hemoglobin from blood for hemoglobin variant research. It is compatible with protocols which require direct surface sampling of dried blood spot (DBS) to identify intact proteins. Pharmaceutical testing of DBS samples from clinical matrices for bioanalytical applications during the drug development process favors DBS technology because of reduced animal consumption, reduced shipping costs, storage at ambient temperature and decreased infection risk and toxicity. With HemoVoid™ Blood Card, the enriched hemoglobin voids in flow-through greater than 98% pure, with less than 30 minute bind/wash/elute protocol. Researchers studying hemoglobin variant (HbS, HbE, HbC, HbD, HbF, HbA1c,Thalassemia, etc) should implement HemoVoid™ Blood Card’s efficient protocol. Mild buffer condition maintains tertiary structure and simple transfer to secondary analysis.

The hemoglobin enriched filtrate could have hemoglobin variants, hemoglobin binding proteins or other analytes optimal for biomarker studies. Eluted fractions contains hemoglobin depleted proteins which can be used for developing and validating LC-MS, proteomic studies including protein identification characterizing low abundance proteins for protein biomarker research and gain a broader understanding of the structural, catalytic and signaling functions of such proteins.

HemoVoid™ features an easy to implement sample preparation protocol. It is disposable, cost-effective and high-throughput compatible for the detection of the variant peptides making it an ideal choice for hemoglobin variants discovery for hemoglobin proteomics research. HemoVoid™ , a silica-based protein enrichment matrix, removes hemoglobin from erythrocyte lysate samples while concentrating low abundance, and/or low molecular weight proteins.

For more information about Hemovoid™ Blood Card, click:.

https://www.biotechsupportgroup.com/node/238

About Biotech Support Group LLC

Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include: AlbuVoid™ for albumin depletion, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Biotech Support Group is the leading developer of sample preparation products for separating and purifying hemoglobin from blood samples. HemoVoid™ is a hemoglobin depletion reagent kit from red blood cells and HemogloBind™ is a hemoglobin capture reagent from hemolyzed serum. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com

Hemoglobin enrichment from dried blood spot (DBS) for hemoglobin variant research

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