Cyclic AMP (cAMP) is the prototypical second messenger. The major receptor in eukaryotic cells, cAMP-dependent Protein Kinase (PKA), is ubiquitous. cAMP affects many different physiological and biochemical processes including the activity of kinases.

cADDis – An elegant means to measure cAMP signaling in real time, in the living cells of your choice.

Save time and money by switching to cADDis, a simple and robust assay for Gs and Gi signaling, without the need for co-factors, cell lysis, or complex liquid handling. cADDis is genetically-encoded and packaged in BacMam for easy transduction into a wide variety of cell types, including primary and iPSC-derived cell lines. Simply add cADDis to your cells and measure robust signals the very next day on standard fluorescence microscopes and automated plate readers.

Gs detection: Kinetics. Dose responses. Bright and reliable signals.

Measure kinetics and dose responses of Gs-mediated cAMP production with large and consistent