AMSBIO has added a new range of GCaMP calcium indicators (jGCaMP7b, jGCaMP7c, jGCaMP7f, jGCaMP7s) to their portfolio of ready-to-use adeno-associated virus (AAV) biosensor products. These viruses encoded biosensors are supplied ready for use for neural activity studies in vivo.

AAV Biosensors provide a snapshot of intracellular interactions, specifically active brain circuitry. Using fluorescent protein-based AAV biosensors allows permanent marking of active cells over short time scales, providing a window into the real-time biochemistry of living cells and whole organisms.

GCaMP is a genetically encoded calcium indicator (GECI) generated from a fusion of the green fluorescent protein (GFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. Upon binding of GECI to Ca2+ there is an induction of a change in fluorescence signal. It is this change in signal that allows for measurement of the action potentials, and other receptor activation events, that trigger Ca2+ fluxes. GCaMP7 are the 7th generation of GCaMP, offering improved engineering for increased signal-to-noise ratio and much faster kinetics, when compared to previous versions, specifically, GCaMP3 and GCaMP5G.

AMSBIO AAV biosensors are introduced to cells, tissues or organisms to detect changes by fluorescent microscopy or spectrum changes. These biosensors permit long-term imaging and can be engineered to specifically target cellular compartments or organelles. Additionally, these biosensors permit signaling pathway exploration or allow the measurement of a biomolecule. They do all this while preserving both spatial and temporal cellular processes.

AMSBIO offers a range of Calcium or Glutamate Biosensor products that come ready-to-use, with a choice of promoter and the ability to include the Cre inducible (FLEx-ON) expression. AMSBIO has packaged these indicators into the most commonly used AAV serotypes (AAV8 and AAV9). Different serotypes are available on request.

For further information on ready-to-use adeno-associated virus (AAV) biosensor products please visit http://www.amsbio.com/aav-biosensors.aspx or contact AMSBIO on +44-1235-828200 / +1-617-945-5033 / [email protected].

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading transatlantic company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products and services for research and development in the medical, nutrition, cosmetics and energy industries. AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics.

For drug discovery research, AMSBIO offers assays, recombinant proteins and cell lines. Drawing upon a huge and comprehensive biorepository, AMSBIO is widely recognised as a leading provider of high-quality tissue specimens (including custom procurement) from both human and animal tissues. The company provides unique clinical grade products for stem cell and cell therapy applications these include high quality solutions for viral delivery (lentivirus, adenovirus and adeno-associated virus) in addition to GMP cryopreservation technology. For further information please visit www.amsbio.com.