Upgraded MassFluidix HC system launched in response to customer feedback, optimizing efficiency and quality of mass photometry experiments

Refeyn’s new MassFluidix 2^nd generation microfluidic chip enables rapid sample dilution and mass photometry measurement in each of its five channels.

 Oxford, UK – Refeyn, the company behind pioneering mass photometry technologies, has taken another step to assure mass photometry is a simpler, more efficient bioanalytics tool with the release of a powerful expansion of its consumables portfolio. The introduction of next-generation microfluidic chips and improvements to Refeyn’s microfluidic system – as well as a new protein calibration standard – will save users time, while ensuring reproducibility across experiments and labs, and supporting a broader range of applications.

To enhance the rapid dilution of highly concentrated samples and improve insight into low-affinity and transient biomolecular interactions, several updates have been made to Refeyn’s popular MassFluidix^® HC microfluidics system. A key addition to this system is a 2^nd generation microfluidic chip which now enables mass photometry measurements in five channels and features even faster sample dilution – in under 37 milliseconds. Usage is simplified with a channel redesign that removes the need for a bubble trap and introduces luer connectors that enable easy connection of tubing to the chip. These ready-to-use chips are also now individually packaged, allowing customers to open just one at a time as needed.

Further improving ease-of-use of the MassFluidix HC, software integration now means that the rapid dilution system can be readily operated directly via Refeyn’s Acquire^MP mass photometer control software. A new automatic microfluidic chip channel detection feature within this software also simplifies workflows by ensuring correct positioning of the chip for each measurement.

The upgraded system’s rapid dilution allows the characterization of samples at high concentration. This is particularly useful as a quality check for techniques that require higher concentrations, such as cryoEM and crystallography, as well as for detecting and determining stoichiometry for weak affinity protein-protein interactions. This has implications for traditionally difficult-to-characterize sample types, such as PROTACs and multi-component complexes; in addition to more common applications, such as antibody-receptor binding, and as an orthogonal technique to other binding characterization techniques.

Additionally, a new mass calibrant joins Refeyn’s portfolio of calibration standards, which ensure maximum precision of mass photometry measurements and minimise hassle for customers. Simple to prepare, Refeyn’s MassFerence^® mass calibration standard range provides standardized references, increasing replicability across experiments, instruments and laboratories. The new MassFerence^® P1 protein standard is introduced for use with Refeyn’s Two^MP and One^MP mass photometers for calibrating measurements of proteins ranging from 90 to 1000 kDa.

Commenting on Refeyn’s growing mass photometry consumables range, Gerry Mackay, CEO, Refeyn, said, “These new products reflect our commitment to responding to customer needs and working to provide the best possible experience for all users of mass photometry technologies. With their introduction, we aim to optimize the efficiency and quality of mass photometry experiments, and making mass photometry useful for a wider range of samples.”

Refeyn’s Director of Consumables, Camille Hetez, added, “We’ve listened to our customers and made many changes and additions to suit their requirements. By using consumables that we’ve developed specifically for Refeyn instruments, our customers can benefit from hassle-free measurements, with less time needed for sample prep and more confidence in their mass photometry biomolecular analysis results.”

To find out more about Refeyn and its novel mass photometry technologies and consumables range, please visit: https://www.refeyn.com

About Refeyn www.refeyn.com

Refeyn Ltd. specializes in the development, production, and distribution of mass photometry technologies for industry and academia. Mass photometry and macro mass photometry are unique bioanalytical technologies pioneered by Refeyn. By quantifying light scattering from particles in solution, mass photometry enables the measurement of molecular mass at the single-particle level while macro mass photometry enables simultaneous measurement of viral vector size and contrast (a proxy for mass).

Spun out of Oxford University in 2018, the company aims to make its technology accessible to all in the scientific community, enabling users to achieve new and better outcomes. Refeyn has been recognized for its unique approach to biomolecular analysis through multiple awards, including the Royal Society of Chemistry Emerging Technologies Competition. Applications of mass photometry include: Characterization of samples of biomolecules and viral vectors, and analysis of protein oligomerization, biomolecular interactions, and macromolecular assemblies. Utilizing the novel technologies of mass photometry and macro mass photometry, Refeyn’s products are capable of accurate analysis of single particles in solution, in their native state, and without the need for labels, opening up new possibilities for bioanalytics.