Simplified Detection of DDK-Tagged Recombinant Proteins

AMSBIO offers a family of anti-tag antibodies to optimize the detection and purification of recombinant tagged proteins. Most of AMSBIO’s anti-tag antibodies are mouse monoclonal antibodies which have been screened and extensively validated for high sensitivity and specificity, in applications including Western Blot, Immunofluorescent Staining, Flow Cytometry and Immunoprecipitation.

The anti-DDK clone 4C5 antibody detects the DDK epitope which is widely used as tag in recombinant proteins and is included in the pCMV6-Entry vector that forms the backbone of the TrueORF cDNA series. It has been used to validate the expression of over 15,000 mammalian recombinant proteins, purify more than 5,000 human recombinant proteins from HEK293 cells and purify immunogens under native conditions for the development of antibodies.

The new conjugated anti-DDK antibodies (linked to HRP, Dylight 488/550**, Phycoerythrin and biotin) have been developed using the highest standards of production procedure and validation. Using these premium quality products researchers now have a simpler way to detect proteins with low expression levels and purify recombinant proteins with high purities and yields.

Founded in 1987, AMS Biotechnology (AMSBIO) is recognized as a leading international provider of unique, innovative products & custom services for life sciences research. The AMSBIO range includes over 23,000 polyclonal & monoclonal antibodies, peptides, recombinant proteins, extracellular matrix, molecular detection reagents, & tissue DNA, RNA, protein & microarray products. Key research areas include: apoptosis, cell invasion & migration, cell signaling, DNA damage, 3D culture, electrophoresis, glycobiology, post-translational modification & stem cell biology.

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