Madrid, Spain and Heidelberg, Germany — SYGNIS AG (Frankfurt: LIO1; ISIN: DE000A1RFM03; Prime Standard) today announced that it has signed a non-exclusive distribution agreement for its proprietary TruePrime™ Single Cell WGA Kit with D-MARK BIOSCIENCES for the North American market.

Under the terms of the agreement, SYGNIS grants D-MARK BIOSCIENCES the non-exclusive rights to promote, market and sell TruePrime™ Single Cell WGA Kit to scientists working in the wide field of molecular biology in Canada. TruePrime™ Single Cell WGA Kit is the first in a row of products of SYGNIS’ TruePrime™ product family based on the combination of the recently discovered DNA primase “TthPrimPol” and the Phi29 polymerase which allows the amplification of total genomic DNA from single cells without the need of random synthetic primers.

FD-MARK BIOSCIENCES is a specialized provider offering a broad portfolio of innovative research reagents as well as state-of-the-art instruments in the wide field of molecular biology including next generation sequencing and gene silencing applications to customers all over Canada.

“We are very pleased about this distribution agreement for our proprietary product with D-MARK BIOSCIENCES, an important door opener to North America. With its wide customer network focused on molecular biology, D-MARK BIOSCIENCES is best positioned to significantly expand our market reach and make our product available in Canada,” Pilar de la Huerta, CEO and CFO of SYGNIS commented. “After rolling out TruePrime™ Single Cell WGA Kit in Europe, North America is the next logical step and a further milestone in our product and commercialization strategy. The high interest in our product we are experiencing encourages us in our efforts to further expand our distribution channels to leverage the benefits of this new technology to researchers working in NGS throughout the world.”

TruePrime™ is the brand name of a revolutionary novel MDA technology and one of the key products in SYGNIS’ portfolio. TruePrime™ is based on the combination of the recently discovered DNA primase “TthPrimPol” and the extremely processive and high-fidelity Phi29 polymerase to amplify uniformly total genomic DNA for a multitude of applications including next generation sequencing and single cell analysis. The extraordinary strand displacement capacity of the Phi29 polymerase allows TthPrimPol to generate new primers on the displaced strands that are extended by Phi29 DNA pol, resulting in exponential isothermal DNA amplification without the need of any synthetic random primers.

The resulting benefits of this innovative approach are enormous and include complete absence of common artifacts linked to the use of oligonucleotides, a reduced amplification bias in genome coverage compared to methods using random synthetic primers, a surpassing reliability as contaminating DNA is not amplified and an exquisite reproducibility when DNA is amplified from single mammalian cells. Moreover, TruePrime™ shows superior sensitivity, is easy to use and works perfectly well with all commonly used NGS platforms such as Illumina and Ion Torrent.