Thermo Fisher Scientific Introduces HPLC Ion Exchange Columns for Protein Charge Variant and AAV Separation Analysis

SAN JOSE, Calif.


Thermo Fisher Scientific is providing biopharmaceutical research laboratories with a new line of ion exchange columns that deliver superior reproducibility, sensitivity and durability for charge-based analysis of therapeutic proteins and separation offull vs. empty adeno-associated virus (AAV) capsids used for gene therapy.




The Thermo Scientific ProPac 3R HPLC column portfolio leverages a novel monodisperse particle platform and state-of-the-art separation power to deliver outstanding lot-to-lot reproducibility and consistent results over the lifecycle of a drug, from research and development to commercialization. The new 3 µm particle size enables a new level of performance with ultra-high resolution of charge variants, improved peak resolution and faster run times.

The portfolio supports strong anion-exchange (SAX) and strong cation-exchange (SCX) column chemistries, each with two column lengths (50mm and 100mm) and two inner diameters (2mm and 4mm).

Key Applications:

Biopharmaceutical laboratories performing charge variant analysis of therapeutic proteins, including monoclonal antibodies, and separation of full versus empty AAV capsids used for gene therapy


  • Compatible with the mobile phases and organic solvents used in biopharmaceutical applications
  • Designed with bio-inert materials to reduce secondary interactions
  • Improved peak-to-valley resolution of charge variants to increase certainty of quantification of critical quality attributes
  • Confidence in the detection and identification of new acidic or basic variants during late-stage development
  • High sample-loading capacity
  • Analytical flexibility for excellent sensitivity and performance under a broad range of pH, temperature and mobile phase compositions as well as MS capabilities


To learn more about the new ProPac 3R HPLC columns, please visit: Charge Variant Analysis HPLC Columns | Thermo Fisher Scientific – US

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science, with annual revenue over $40 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, increasing productivity in their laboratories, improving patient health through diagnostics or the development and manufacture of life-changing therapies, we are here to support them. Our global team delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services, Patheon and PPD. For more information, please visit


Media Contact Information

Piatra Marani

Thermo Fisher Scientific

[email protected]

Chanel Hastings


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