SAN DIEGO, — Trovagene, Inc. (Nasdaq: TROV), (Nasdaq: TROVU),a developer of trans-renal molecular diagnostics, announced the full exercise of the over-allotment option granted to the underwriters to purchase 172,500 additional units, at a public offering price of $8.00 per unit, in connection with its previously announced underwritten public offering of 1,150,000 units, bringing total gross proceeds from the offering to $10,580,000.

On June 14, 2012, the holders of the Company’s units may elect to separately trade the common stock and warrants underlying the units on The NASDAQ Capital Market under the symbols “TROV” and “TROVW”, respectively. The warrants will be exercisable at an exercise price of $5.32 per share upon separation of the units and will expire on June 4, 2017. Those units not separated will continue to trade on The NASDAQ Capital Market under the symbol “TROVU”.

Aegis Capital Corp. acted as the sole book-running manager for the offering.

Summer Street Research Partners and Brean Murray, Carret & Co. acted as co-managers for the offering.

A registration statement on Form S-1 relating to the shares was filed with the Securities and Exchange Commission and is effective. Copies of the final prospectus relating to the offering may be obtained from the offices of Aegis Capital Corp., Prospectus Department, 810 Seventh Avenue, 18th Floor, New York, NY, 10019, telephone: 212-813-1010 or email: prospectus@aegiscap.com, or from the above-mentioned SEC website.

About Trovagene, Inc.

Headquartered in San Diego, California, Trovagene is developing its patented technology for the detection of transrenal DNA and RNA, short nucleic acid fragments, originating from normal and diseased cell death that cross the kidney barrier and can be detected in urine.

Trovagene has a dominant patent position as it relates to transrenal molecular testing. It has U.S. and European patent applications and issued patents that cover testing for HPV and other infectious diseases, cancer, transplantation, prenatal and genetic testing. In addition, it owns worldwide rights to nucleophosmin-1 (NPM1), an informative biomarker for acute myeloid leukemia (AML) and mutations in the SF3B1 gene, which have been shown to be associated with chemotherapy response in CLL (chronic lymphocytic leukemia) patients.