TwistDx’s recently launched range of liquid format Recombinase Polymerase Amplification (RPA) kits are ideal for rapid testing applications and high throughput laboratory use. This isothermal DNA/RNA amplification technology represents a versatile solution for nucleic acid detection, offering the same specificity as PCR but much faster results – typically within three to 10 minutes.

The newly available TwistAmp^® Liquid kits are designed to simplify integration of RPA technology into existing laboratory workflows, offering a faster and more user-friendly alternative to PCR – potentially using the same primers – without the need for a thermocycler. The liquid format also simplifies the development of miniaturised or custom assay configurations and the creation bulk master mixes, as well as allowing users to vary the ratios of individual assay components to optimise their workflows.

Dr Niall Armes, CEO of TwistDx, commented: “The ever growing number of peer-reviewed publications using our technology demonstrates the benefits of RPA as a genuine alternative to traditional PCR techniques. The recent launch of our TwistAmp Liquid kits represents the next step in the development of this technology, helping scientists across a much broader range of applications – from laboratory diagnostics to environmental testing – to take advantage of the speed and sensitivity our approach offers.”

To learn more about RPA technology and the TwistDx range, visit www.twistdx.co.uk  

About TwistDx

TwistDx is a world leader in the development and sale of mobile and point-of-use assays using a unique DNA detection technology, Recombinase Polymerase Amplification (RPA). This technology offers PCR-like sensitivity in just 10 minutes, without the need of a thermocycler. RPA has been used to develop a range of innovative research assays in for food safety and authentication, animal health, agriculture, environmental testing, biodefence and life sciences research.

RPA replaces PCR with a rapid isothermal enzymatic process, using a recombinase which pairs oligonucleotide primers to template DNA and a polymerase to synthesize daughter strands. RPA products can be detected by a variety of means including electrophoresis, lateral flow strips or real-time fluorescence detection. Available in the form of TwistAmp™ kits, RPA is easy to operate using pre-formulated stable reagents, with minimal sample processing and no requirement for a thermocycler. Samples can be tested on- or off-site, with results available within 15 minutes of sample collection, avoiding the time consuming and costly process of sending samples to centralised laboratories. Read over 200 peer-reviewed publications from users benefiting from the incredible speed, simplicity and portability of RPA at www.twistdx.co.uk/publications.