Waters Expands Size Exclusion Chromatography Columns to include ACQUITY Premier and XBridge Premier 125Å SEC Columns for Biotherapeutic Peptides and Small Proteins

WHO: Waters Corporation, Milford, Massachusetts, USA

WHAT: Waters Corporation (NYSE:WAT) has expanded its size-exclusion chromatography product line to include new ACQUITY™ and XBridge™ Premier 125Å SEC Columns and Guard featuring MaxPeak™ High Performance Surfaces (HPS) Column hardware.

The new ACQUITY Premier 125Å SEC Columns provide sized-based separations of peptides and small proteins with 15% improvement in resolution and 6x faster separation compared to existing HPLC-based SEC columns.[i]   In addition, the ACQUITY Premier 125Å SEC 1.7 µm Column enables a 2-fold improvement in insulin high molecular weight product (HMWP) recovery when compared to use of an alternative UHPLC-based 125Å SEC column in a well-controlled experiment.[ii]

The new columns incorporate:

  • MaxPeak High Performance Surfaces [HPS] Technology and manufacturing process, which enables “out-of-the-box” performance on a new SEC column without need for column conditioning.
  • Application-specific, quality control batch-testing with an insulin and small protein mixture to ensure unparalleled column-to-column and batch-to-batch performance reproducibility.
  • 1.7 µm and 2.5 µm ethylene bridged hybrid (BEH™) particles with diol bonding and 125Å  pores optimized for separation of peptides and proteins from approximately 1,000 – 80,000 Daltons.

Why: Scientists working on the development and manufacture of biotherapeutic peptides like GLP-1 receptor agonists need consistent and accurate measurements to increase confidence in the safety, efficacy, and quality of their products. The ACQUITY Premier and XBridge Premier 125Å SEC Columns and Guards offer an out-of-the-box ACQUITY and XBridge Premier SEC Columns and Guard Featuring MaxPeak HPS Column Hardware for higher resolution of size variants and high molecular weight product (HMWP) quantitation by either SEC or SEC-MALS due to the incorporation of MaxPeak High Performance Surfaces. In addition, the columns are suitable for use on existing UHPLC and HPLC systems, making them accessible from drug discovery through to product release.

With the new MaxPeak Premier 125Å SEC Columns, biopharmaceutical scientists can speed up time to results and obtain more consistent measurements across multiple LC platforms

Additional Resources:

ABOUT: Waters Corporation (NYSE: WAT), a global leader in analytical instruments and software, has pioneered chromatography, mass spectrometry, and thermal analysis innovations serving the life, materials, food, and environmental sciences for over 65 years. With approximately 7,500 employees worldwide, Waters operates directly in 35 countries, including 14 manufacturing facilities, and with products available in more than 100 countries.

[i] The ACQUITY Premier SEC 125Å, 1.7 µm, 4.6 x 150 mm column provides 15% higher resolution and 6x faster separations than the Waters Insulin HMWP 125Å, 10 µm, 7.8 x 300 mm column when separating human insulin using USP <121.1> without prior column conditioning.

[ii] The ACQUITY Premier SEC 125Å, 1.7 µm, 4.6 x 150 mm column provides a 2x increase in HMWP recovery following analysis of a human insulin sample when compared to a commercially available SEC 120Å, 1.9 µm, 4.6 x 150 mm in a well-controlled experiment.

 

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