The OptoSeq 3′ mRNA Library Kit allows users of Berkeley Lights’ platform to efficiently prepare cells of interest for genomic sequencing opening the door to directly link phenotype to genotype using a single system.

EMERYVILLE, Calif.— Today, Berkeley Lights, a leader in cell selection, announced their new OptoSeq 3′ mRNA Library Kit enabling users to directly link phenotype to genotype.  Using the OptoSeqTM 3′ mRNA Library Kit, Beacon and Lightning system customers can now capture and link genomic information to the single cell phenotypic data to drive further insight into cellular behaviors to accelerate the discovery, development and production of cell-based products.

The OptoSeq 3′ mRNA Library Kit significantly expands the capabilities of the Berkeley Lights platform.  Users of the company’s T cell workflow will be able to understand why and when T cells get activated.  Those running a cell line development workflow will be able to characterize the transcriptome of the highest producing clones.

“After researchers culture and assay cells on the Beacon or Lightning instruments and identify the cells of interest, they typically want to run those select cells through RNA sequencing to better understand their genes to explicitly link the observed phenotype to the underlying genotype,” said John Proctor, Ph.D., Senior Vice President of Marketing at Berkeley Lights. “With the new OptoSeq 3′ mRNA Library Kit, customers can now use the Berkeley Lights platform to refine library prep, allowing users to consolidate workflows and complete the cell journey on one system.”

The OptoSeq 3′ mRNA Library Kit includes reagents, primers, probes, and enzymes to prepare and label individual cells for cDNA sequencing. It is supported by the Berkeley Lights PrimeSeq software integrated bioinformatics pipeline that analyzes genes and generates data reports.  The Berkeley Lights platform enables the capture of robust phenotypic data single-cell by single-cell, over time, allowing our customers to gain deep information about cellular populations and subpopulations. These capabilities uniquely enable customers to perform highly parallel and rapid functional validation of thousands of cells at the single cell level.  Users can now capture, lyse, and barcode genetic information from select cells on a single OptoSelect chip, conduct reverse transcription, create cDNA libraries, and bioinformatically link sequencing results back to the unique cell identifiers using a single innovative platform. And in the future, users of the company’s synthetic biology workflows will be able to use the data to inform cellular models enabling better organism design.

You can find more information here: https://www.berkeleylights.com