New 1-Step RT-qPCR Master Mix from Thermo Fisher Scientific helps increase productivity and performance with automation-ready formula


Overview: Labs incorporating automation into their qPCR workflows need assurance that their reagents are stable during reaction setup and testing. However, existing 1-Step RT-qPCR master mixes experience lower sensitivity, specificity, and consistency when assembled reactions are left at room temperature for extended periods. Unlike other 1-Step RT-qPCR Master Mixes available on the market, the new Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix from Thermo Fisher Scientific maintains performance at room temperature for a full work day (>8hours), providing more flexibility for labs to incorporate 1-Step RT-qPCR into their automated workflow.

The TaqPath DuraPlex 1-Step RT-qPCR Master Mix is now available for biopharma and clinical diagnostics labs that need a rapid, sensitive, and reproducible method for developing and testing high-throughput molecular diagnostic or bioprocess quality control assays. This benchtop-stable, single-tube master mix is optimized for detection of virus and bacterial pathogens even in the presence of challenging PCR inhibitors. Labs can now confidently automate their 1-Step RT-qPCR workflows, without loss of sensitivity, specificity, or reproducibility, with the ability to multiplex up to 6 targets in a single reaction.

“Overall, we were quite pleased with the performance of TaqPath DuraPlex 1-Step RT-qPCR Master Mix and found that it performed considerably better than a similar on-market formulation we tested in conjunction. Specifically, we found the product to perform significantly better in both inhibitor tolerance and in-channel multiplexing, especially at low copy number,” said Alison Todd, AM, FTSE, PhD, Chief Scientific Officer & Founder, SpeeDx Pty Ltd.

Key Applications:
  • Pharma/Biotech R&D and Bioanalysis Labs
  • Clinical diagnostic testing labs
  • Molecular diagnostic kit manufacturers


  • Automates workflows: allows customers to confidently automate their molecular diagnostic and gene expression analysis workflows with liquid handling instrumentation by providing over 8 hours of room temperature stability of assembled reactions, without loss of sensitivity, specificity, or reproducibility;
  • Optimizes high order multiplexing: 4X concentrated formulation is capable of multiplexing 6 targets in a single well with exceptional sensitivity and specificity, streamlining qPCR assay development and testing turnaround time;
  • Improves handling: provides lower viscosity and more flexible in-use and storage characteristics to help improve automation performance and compatibility;
  • Increases confidence:  highly tolerant to qPCR inhibitors such as VTM, stool, blood, and urine, enabling assay performance even when working with challenging sample types
  • Helps ensure reproducibility:  a general purpose reagent labeled “For Laboratory Use” and manufactured in an ISO 13485-certified and FDA-registered facility, which adheres to cGMP; all lots are functionally tested to help ensure lot-to-lot reproducibility and consistent performance

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* For Laboratory Use. It is the customer’s responsibility to ensure that the performance of the product is suitable for customers’ specific uses or applications.

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