Thermo Scientific Glacios 2 Cryo-TEM with Low-Energy-Spread Cold FEG (E-CFEG)

Product: Thermo Scientific Glacios 2 Cryo-TEM with E-CFEG

Product Image: 

A reconstruction of apoferritin at 1.5 Å resolution from data collected over seven hours using the Thermo Scientific Glacios 2 Cryo-TEM with the Thermo Scientific Falcon 4i Direct Electron Detector, Selectris X Imaging Filter, E-CFEG, and fringe-free imaging

Product Overview:

The Thermo Scientific™ E-CFEG, a low-energy-spread cold field emission source launched as a value added component for the company’s highest-powered 300kV cryo-transmission electron microscope (cryo-TEM), is now also an option for the 200kV Thermo Scientific Glacios™ 2 Cryo-TEM.

This expanded offering is designed to help propel accessibility to cryo-electron microscopy (cryo-EM) techniques that boost resolution while maintaining ease-of-use and productivity capabilities. With a narrow energy spread to support high contrast images, the addition of the E-CFEG to the Glacios 2 Cryo-TEM enables near-atomic structural determination of complex proteins and macromolecules, and therefore, accelerated cryo-EM research and drug discovery processes using a lower accelerating voltage.

New data from Thermo Fisher Scientific using this combination demonstrates its ability to generate a 1.5 Å resolution structure of Apoferritin. This is currently the highest resolution structure generated from a commercially available cryo-TEM operating at 200kV.

In a package that reduces workflow complexity for users of various experience levels for single particle analysis, cryo-electron tomography and Micro-ED workflows, the platform add-on also includes AI-enabled algorithms for data collection and automated microscope alignments for increased ease of use to enable users to produce consistently excellent data.

Key Applications:

For structural biologists in biopharmaceutical and academic research who need high quality structural data at high throughput with more automation for improved efficiency and ease of use.


  • Powerful imaging potential to enable structure determination with improved resolution at <2A.
  • Rapid data acquisition and data analysis with automated features to steer data collection, reduce idle time and enable users of a wide range of expertise levels to obtain high quality cryo-EM data.
  • A smooth imaging experience with reduced effort due to a streamlined interface with built-in self-correction function to assist with microscope alignment and traffic light functionality for microscope readiness.

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Company Background: 

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science, with annual revenue over $40 billion. Our Mission is to enable our customers to make the world healthier, cleaner and safer. Whether our customers are accelerating life sciences research, solving complex analytical challenges, increasing productivity in their laboratories, improving patient health through diagnostics or the development and manufacture of life-changing therapies, we are here to support them. Our global team delivers an unrivaled combination of innovative technologies, purchasing convenience and pharmaceutical services through our industry-leading brands, including Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific, Unity Lab Services, Patheon and PPD. For more information, please visit

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